Deep sequencing of the viroid-derived small RNAs accumulating in peach infected by two symptomatic variants of Peach latent mosaic viroid. (Comunicazione a convegno)

Type

• Prodotto della ricerca (Classe)
• Comunicazione a convegno (Classe)

Label

• Deep sequencing of the viroid-derived small RNAs accumulating in peach infected by two symptomatic variants of Peach latent mosaic viroid. (Comunicazione a convegno) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• Di Serio F., Gisel A., Navarro B., Delgado S., Martínez de Alba Á.E., Donvito G. and R. Flores (2009)
  Deep sequencing of the viroid-derived small RNAs accumulating in peach infected by two symptomatic variants of Peach latent mosaic viroid.
  in 21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Nuestadt (Germany)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Di Serio F., Gisel A., Navarro B., Delgado S., Martínez de Alba Á.E., Donvito G. and R. Flores (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Northern-blot hybridization of nucleic acid preparations from plants infected by representative members of both viroid families has revealed the presence of 21-24 nt viroid-derived small RNAs (vd-sRNAs) with the structural properties of the small interfering RNAs (siRNAs), the characteristic effectors of RNA silencing . These results strongly support that viroids are elicitors and targets the RNA silencing machinery of their hosts. Moreover, conventional sequencing of the vd-sRNAs from two members of the family Pospiviroidae and one of the Avsunviroidae has confirmed that they are predominantly of 21 and 22 nt and (+) polarity. This approach, however, generates incomplete datasets and, therefore, the ensuing conclusions may be biased. To overcome this limitation we have subjected to deep sequencing the vd-sRNAs accumulating in GF-305 peach seedlings infected by two molecular variants of Peach latent mosaic viroid (PLMVd), one causing peach mosaic and the other peach calico. The analysis of the resulting PLMVd-sRNAs permits to draw inferences about the dicer-like enzymes catalyzing their genesis and the RNA substrates upon which they act, as well as to assess whether some specific PLMVd-sRNAs containing the pathogenicity determinant of peach calico may be involved in symptom development. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Tecnologie Biomediche del CNR, Bari, Italy. Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC),Valencia, Spain. Istituto Nazionale di Fisica Nucleare, Bari, Italy (literal)

Titolo

• Deep sequencing of the viroid-derived small RNAs accumulating in peach infected by two symptomatic variants of Peach latent mosaic viroid. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• BEATRIZ NAVARRO RAMIREZ (Persona)
• FRANCESCO DI SERIO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Deep sequencing of the viroid-derived small RNAs accumulating in peach infected by two symptomatic variants of Peach latent mosaic viroid." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• BEATRIZ NAVARRO RAMIREZ (Persona)
• FRANCESCO DI SERIO (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Insieme di parole chiave di

• Keywords of "Deep sequencing of the viroid-derived small RNAs accumulating in peach infected by two symptomatic variants of Peach latent mosaic viroid." (Insieme di parole chiave)