Deep sequencing of viroid-derived small RNAs accumulating in peach plants infected by Peach latent mosaic viroid. (Comunicazione a convegno)

Type

• Prodotto della ricerca (Classe)
• Comunicazione a convegno (Classe)

Label

• Deep sequencing of viroid-derived small RNAs accumulating in peach plants infected by Peach latent mosaic viroid. (Comunicazione a convegno) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• F. Di Serio, A. Gisel, B. Navarro, S. Delgado, Á.E. Martinez de Alba, G. Donvito and R. Flores (2009)
  Deep sequencing of viroid-derived small RNAs accumulating in peach plants infected by Peach latent mosaic viroid.
  in XV Congresso della Società Italiana di Patologia Vegetale, Locorotondo (Bari)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• F. Di Serio, A. Gisel, B. Navarro, S. Delgado, Á.E. Martinez de Alba, G. Donvito and R. Flores (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Viroids are infectious non-protein-coding RNAs that may cause plant diseases by a mechanism still unknown. A recent hypothesis has linked viroid pathogenesis to RNA silencing, a sequence-specific degradation mechanism mediated by small RNAs of 21-24 nt (sRNAs). This hypothesis, which has not been conclusively proved, relies on the detection of viroid-derived 21-24 nt sRNAs (vd-sRNAs) in infected tissues and predicts that some of them drive down-regulation of host gene(s) eliciting the pathogenic process. Here, we exploited the experimental system formed by the chloroplast-replicating Peach latent mosaic viroid infecting its natural host peach to further dissect the biogenesis of vd-sRNAs and the role that they may have in viroid pathogenesis. To this aim, vd-sRNAs accumulating in peach leaves infected by two PLMVd variants, which induce an albino phenotype (peach calico, PC) and a typical mosaic, were characterized by deep sequencing (Solexa-Illumina). Our results suggest the involvement of cytoplasmatic DICER-like enzymes in the genesis of the vd-sRNAs of 21 and 22 nt, using as templates the genomic PLMVd RNA of both polarities or the dsRNAs synthesized by a host RNA-dependent RNA polymerase. Moreover, since vd-sRNAs from the insertion of 12-14 nt wherein the PC determinant maps are almost undetectable, it is unlikely that they could be involved in PC, thus suggesting that a mechanism different from that proposed operates in PLMVd pathogenesis. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Tecnologie Biomediche del CNR, Bari, Italy. Instituto de Biologia Molecular y Celular de Plantas (UPV-CSIC),Valencia, Spain. Istituto Nazionale di Fisica Nucleare, Bari, Italy (literal)

Titolo

• Deep sequencing of viroid-derived small RNAs accumulating in peach plants infected by Peach latent mosaic viroid. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• BEATRIZ NAVARRO RAMIREZ (Persona)
• FRANCESCO DI SERIO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Deep sequencing of viroid-derived small RNAs accumulating in peach plants infected by Peach latent mosaic viroid." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• BEATRIZ NAVARRO RAMIREZ (Persona)
• FRANCESCO DI SERIO (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Insieme di parole chiave di

• Keywords of "Deep sequencing of viroid-derived small RNAs accumulating in peach plants infected by Peach latent mosaic viroid." (Insieme di parole chiave)