The molecular biology of two Tombusvirus-associated satellite RNAs (Comunicazione a convegno)

Type
Label
  • The molecular biology of two Tombusvirus-associated satellite RNAs (Comunicazione a convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Rubino, l., Russo, M. (2009)
    The molecular biology of two Tombusvirus-associated satellite RNAs
    in XV Convegno Nazionale SIPaV, Locorotondo (Bari)
    (literal)
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  • Rubino, l., Russo, M. (literal)
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  • pubblicato in Journal of Plant Pathology, 91, S4.39 (literal)
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  • Plant virus infections are often associated with subviral RNA molecules, constituted by defective interfering (DI) or satellite (sat) RNAs, which are both totally dependent on helper virus-encoded factors for their replication. DI RNAs are shortened forms of the viral genome, generated by discontinuities of the viral replicase and deprived of all viral genes required for replication. SatRNAs share little or none sequence homology with the helper virus genome and are dispensable for virus propagation. Three satRNAs associated with members of the genus Tombusvirus have been described so far. They are linear, single-stranded non-coding RNAs c. 600-800 nt in size, with no sequence homology with the helper virus, except for a 50 nt stretch present in the 5’ non coding region of all tombusviral genomes and DI RNAs. The well characterized satRNA associated with Cymbidium ringspot virus (CymRSV) does not attenuate virus-induced symptom expression, is not replicated in protoplasts from transgenic Nicotiana benthamiana plants expressing the viral replicase, and is the target of virus induced-RNA silencing. By contrast, satB10, a different satRNA associated with natural Tomato bushy stunt virus (TBSV) infections, attenuates symptom expression. We show that the expression of viral replicase proteins in transgenic protoplasts is sufficient for satB10 replication, suggesting that different strategies are deployed for the replication of different satRNAs. We also describe the biological properties of satRNA L, a novel satRNA associated with TBSV. In particular, we show that satRNA L and B10 are both initiators and target of RNA silencing. (literal)
Titolo
  • The molecular biology of two Tombusvirus-associated satellite RNAs (literal)
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