Studies on the Replication of Tombusvirus Satellite RNAs (Abstract/Poster in convegno)

Type
Label
  • Studies on the Replication of Tombusvirus Satellite RNAs (Abstract/Poster in convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • RUBINO L. & RUSSO M. (2010)
    Studies on the Replication of Tombusvirus Satellite RNAs
    in Silencing Mechanisms in Plants meeting, Santa Fe, New Mexico
    (literal)
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  • RUBINO L. & RUSSO M. (literal)
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  • Plant virus infections are often associated with subviral RNA molecules, constituted by defective interfering (DI) or satellite (sat) RNAs. Both these RNAs are totally dependent on helper virus-encoded factors for their replication. DI RNAs are shortened forms of the viral genome, generated by discontinuities of the viral replicase and deprived of all viral genes required for replication. SatRNAs share little or no sequence homology with the helper virus genome and are dispensable for virus propagation. Three satRNAs associated with members of the genus Tombusvirus have been described so far. They are linear, single-stranded non-coding RNAs c. 600-800 nt in size, with sequence homology with the helper virus, limited to a 50 nt stretch in the 5’ non coding region of all tombusviral genomes and DI RNAs. The well characterized satRNA associated with Cymbidium ringspot virus (CymRSV) does not attenuate virus-induced symptom expression, is not replicated in protoplasts from transgenic Nicotiana benthamiana plants expressing the viral replicase, and is the target of virus induced-RNA silencing. In this work the biological properties of satRNA L, a novel satRNA associated with TBSV are described. The relevance of the genomic ORF1 product for satRNA L replication was investigated using different hybrid tombusviruses as helper. SatRNA L and B10, a different satRNA associated with natural Tomato bushy stunt virus (TBSV) infections, were shown to be both initiators and target of RNA silencing. (literal)
Titolo
  • Studies on the Replication of Tombusvirus Satellite RNAs (literal)
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