http://www.cnr.it/ontology/cnr/individuo/prodotto/ID15898

NEMO-binding domain peptide inhibits proliferation of human melanoma cells. (Articolo in rivista)

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NEMO-binding domain peptide inhibits proliferation of human melanoma cells. (Articolo in rivista) (literal)

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Ianaro A, Tersigni M, Belardo G, Di Martino S, Napolitano M, Palmieri G, Sini M, Maio AD, Ombra M, Gentilcore G, Capone M, Ascierto M, Satriano RA, Farina B, Faraone-Mennella M, Ascierto PA, Ialenti A. (2009)
NEMO-binding domain peptide inhibits proliferation of human melanoma cells.
in Cancer letters (Print)
(literal)

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Ianaro A, Tersigni M, Belardo G, Di Martino S, Napolitano M, Palmieri G, Sini M, Maio AD, Ombra M, Gentilcore G, Capone M, Ascierto M, Satriano RA, Farina B, Faraone-Mennella M, Ascierto PA, Ialenti A. (literal)

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a Department of Experimental Pharmacology, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy b Department of Biology, University of Rome Tor Vergata, Rome, Italy c Medical Oncology and Innovative Therapy National Tumor Institute, Fondazione Pascale, Naples, Italy d Clinical Immunology National Tumor Institute, Fondazione Pascale, Naples, Italy e Unit of Cancer Genetics, Institute of Biomolecular Chemistry, C.N.R., Sassari, Italy f Department of Biochemical and Biochemistry, Federico II University of Naples, Italy g Institute of Food Sciences, C.N.R., Avellino, Italy h Clinical Dermatology, Second University of Naples, Italy (literal)

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NEMO-binding domain peptide inhibits proliferation of human melanoma cells. (literal)

Abstract

Melanoma is the most aggressive form of skin cancer, it originates from melanocytes and its incidence has increased in the last decade. Recent advances in the understanding of the underlying biology of the progression of melanoma have identified key signalling pathways that are important in promoting melanoma tumourigenesis, thus providing dynamic targets for therapy. One such important target identified in melanoma tumour progression is the Nuclear Factor-jB (NF-jB) pathway. In vitro studies have shown that NF-jB binding is constitutively elevated in human melanoma cultures compared to normal melanocytes. It has been found that a short cell-permeable peptide spanning the IKK-b NBD, named NBD peptide, disrupted the association of NEMO with IKKs in vitro and blocked TNFa-induced NF-jB activation in vivo. In the present study we investigated the effect of the NBD peptide on NF-jB activity and survival of A375 human melanoma cells. We found that NBD peptide is able to inhibit the proliferation of A375 cells, which present constitutively elevated NF-jB levels. Inhibition of cell proliferation by NBD peptide was associated with direct inhibition of constitutive NF-jB DNA-binding activity and induction of apoptosis by activation of caspase-3 as confirmed by the cleavage and consequently inactivation of poly (ADP ribose) polymerase (PARP-1) known as the best marker of this process. (literal)

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