Observations on the variation of the ribosomal and elongation factor genes in populations of Meloidogyne spp. (Contributo in atti di convegno)

Type
Label
  • Observations on the variation of the ribosomal and elongation factor genes in populations of Meloidogyne spp. (Contributo in atti di convegno) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Rosso L.C., Maresca di Serracapriola M.T. (2007)
    Observations on the variation of the ribosomal and elongation factor genes in populations of Meloidogyne spp.
    in XXXIX Annual Meeting of ONTA, Cordoba (Argentina)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Rosso L.C., Maresca di Serracapriola M.T. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • Nematropica, 37(2), 185. Proceedings of the XXXIX Annual Meeting of ONTA, 29 October-2 November 2007, Villa Carlos Paz, Cordoba, Argentina. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Genomic DNA sequences from eight populations of Meloidogyne spp. from Italy, Spain, Argentina and Chile were obtained by PCR amplification using DNA from single females or isogenic lines. The nuclear gene coding for the elongation factor—1alfa (EF-1alfa), involved in the ribosomial translation, and the SSU rRNA genes were selected for analysis. At least three different variants of the EF-1alfa gene sequence were obtained from PCR products of four Italian populations of M. incognita. The sequences also included introns of variable length. The coding region blast analysis showed high levels of similarity with putative ESTs of the M. incognita EF-1alfa genes available in GenBank. Ribosomal DNA sequences of the same populations also showed nucleotide variations which mirrored different ribosomal sequence data available for the same species. Two variations of the EF-1alfa sequence were also found in the RKN population from Spain, identified through ribosomal data as M. javanica. Both sequences allowed discrimination of the latter species from the M. incognita Italian populations. An EF-1alfa sequence from the Chilean RKN population showed significant differences from the other amplified products, whereas the Argentinian population showed close similarities for both genes with the Italian and the Genbank M. incognita sequences. All variants of the EF-1alfa ESTs identified showed a high conservation of the corresponding amino acid sequences. Data suggest that a combination of the EF-1alfa and ribosomal variants may reveal specific speciation patterns useful to discriminate M. incognita from other Meloidogyne spp. by means of molecular procedures. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • - CNR, Ist. Protezione Piante - Sez. di Bari (literal)
Titolo
  • Observations on the variation of the ribosomal and elongation factor genes in populations of Meloidogyne spp. (literal)
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