Cryopreserved semen in ecotoxicological bioassays: Sensitivity and reliability of cryopreserved Sparus aurata spermatozoa (Articolo in rivista)

Type
Label
  • Cryopreserved semen in ecotoxicological bioassays: Sensitivity and reliability of cryopreserved Sparus aurata spermatozoa (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ecoenv.2012.07.024 (literal)
Alternative label
  • Fabbrocini, A., D'Adamo, R., Del Prete, F., Langellotti, A.L., Rinna, F., Silvestri, F., Sorrenti, G., Vitiello, V., Sansone, G (2012)
    Cryopreserved semen in ecotoxicological bioassays: Sensitivity and reliability of cryopreserved Sparus aurata spermatozoa
    in Ecotoxicology and environmental safety; Academic Press Inc., Elsevier Science, San Diego (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Fabbrocini, A., D'Adamo, R., Del Prete, F., Langellotti, A.L., Rinna, F., Silvestri, F., Sorrenti, G., Vitiello, V., Sansone, G (literal)
Pagina inizio
  • 293 (literal)
Pagina fine
  • 298 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 84 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Ist Sci Marine, I-71010 Foggia, Italy; Univ Naples Federico II, Dipartimento Sci Biol, I-80134 Naples, Italy; Univ Naples Federico II, Ctr Interdipartimentale Ric Gest Risorse Idrobiol, I-80055 Portici, NA, Italy (literal)
Titolo
  • Cryopreserved semen in ecotoxicological bioassays: Sensitivity and reliability of cryopreserved Sparus aurata spermatozoa (literal)
Abstract
  • The aim of this study was to evaluate the feasibility of using cryopreserved S. aurata semen in spermiotoxicity tests. Cryopreservation is a biotechnology that can provide viable gametes and embryos on demand, rather than only in the spawning season, thus overcoming a limitation that has hindered the use of some species in ecotoxicological bioassays. Firstly, the sperm motility pattern of cryopreserved semen was evaluated after thawing by means of both visual and computer-assisted analyses. Motility parameters in the cryopreserved semen did not change significantly in the first hour after thawing, meaning that they were maintained for long enough to enable their use in spermiotoxicity tests. In the second phase of the research, bioassays were performed, using cadmium as the reference toxicant, in order to evaluate the sensitivity of cryopreserved S. aurata semen to ecotoxicological contamination. The sensitivity of the sperm motility parameters used as endpoints (motility percentages and velocities) proved to be comparable to what has been recorded for the fresh semen of other aquatic species (LOECs from 0.02 to 0.03 mg L-1). The test showed good reliability and was found to be rapid and easy to perform, requiring only a small volume of the sample. Moreover, cryopreserved semen is easy to store and transfer and makes it possible to perform bioassays in different sites or at different times with the same batch of semen. The proposed bioassay is therefore a promising starting point for the development of toxicity tests that are increasingly tailored to the needs of ecotoxicology and environmental quality evaluation strategies. (literal)
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