The dosage of Patz1 modulates reprogramming process (Articolo in rivista)

Type
Label
  • The dosage of Patz1 modulates reprogramming process (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1038/srep07519 (literal)
Alternative label
  • Ma, Hui; Ow, Jin Rong; Tan, Bobby Cheng Peow; Goh, Ziyi; Feng, Bo; Loh, Yuin Han; Fedele, Monica; Li, Hu; Wu, Qiang (2014)
    The dosage of Patz1 modulates reprogramming process
    in Scientific reports (Nature Publishing Group)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ma, Hui; Ow, Jin Rong; Tan, Bobby Cheng Peow; Goh, Ziyi; Feng, Bo; Loh, Yuin Han; Fedele, Monica; Li, Hu; Wu, Qiang (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 4 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 12 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • National University of Singapore; Genome Inst Singapore; Chinese University of Hong Kong; Inst Mol & Cell Biol; Consiglio Nazionale delle Ricerche (CNR); Mayo Clin (literal)
Titolo
  • The dosage of Patz1 modulates reprogramming process (literal)
Abstract
  • The acquisition of pluripotent cells can be achieved by combined overexpression of transcription factors Oct4, Klf4, Sox2 and c-Myc in somatic cells. This cellular reprogramming process overcomes various barriers to re-activate pluripotency genes and re-acquire the highly dynamic pluripotent chromatin status. Many genetic and epigenetic factors are essentially involved in the reprogramming process. We previously reported that Patz1 is required for maintenance of ES cell identity. Here we report that Patz1 plays an inhibitory role in OKSM-induced reprogramming process since more iPS colonies can be induced from Patz1(+/-) MEFs than wild type MEFs; while the addition of Patz1 significantly repressed reprogramming efficiency. Patz1(+/-) MEFs can surpass the senescence barrier of Ink4a/Arf locus, thus enhancing iPS colonies formation. Moreover, Patz1(+/-) MEFs displayed higher levels of acetylated histone H3, H3K4me2, H3K4me3, H3K36me3 and lower levels of histone H3K9me3 and HP1 alpha, indicating that heterozygous knockout of Patz1 results in a globally open chromatin which is more accessible for transcriptional activation. However, Patz1(-/-) MEFs gave the lowest reprogramming efficiency which may result from cell senescence trigged by up-regulated Ink4a/Arf locus. Together, we have demonstrated that the dosage of Patz1 modulates reprogramming process via significantly influencing cell senescence, proliferation and chromatin structure. (literal)
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