Bienzyme amperometric probes for choline and choline esters assembled with nonconducting electrosynthesized polymers. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Bienzyme amperometric probes for choline and choline esters assembled with nonconducting electrosynthesized polymers. (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/1521-4109 (literal)

Alternative label

• Curulli, Antonella; Dragulescu, Simona; Cremisini, Carlo; Palleschi, Giuseppe (2001)
  Bienzyme amperometric probes for choline and choline esters assembled with nonconducting electrosynthesized polymers.
  in Electroanalysis (N.Y.N.Y.); Wiley-VCH - Weinheim, Wheinheim (Germania)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Curulli, Antonella; Dragulescu, Simona; Cremisini, Carlo; Palleschi, Giuseppe (literal)

Pagina inizio

• 236 (literal)

Pagina fine

• 242 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 13 (literal)

Rivista

• Electroanalysis (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• pubblicazione scientifica nuovo metodo ad inibizione enzimatica analisi pesticidi (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• II Università di Roma Tor Vergata ENEA Casaccia CNR ISMN Roma (literal)

Titolo

• Bienzyme amperometric probes for choline and choline esters assembled with nonconducting electrosynthesized polymers. (literal)

Abstract

• Different nonconducting polymers have been synthesized on the surface of a platinum (Pt) electrode to assemble fast-response and sensitive amperometric biosensors for choline, butyrylcholine, and acetylcholine, based on choline oxidase (ChOx) and acetylcholinesterase (AchE) or butyrylcholinesterase (BuchE), co-immobilized by crosslinking with bovine serum albumin (BSA) and glutaraldehyde (GLT). Also the electropolymn. conditions as scan rate and time were optimized for each monomer in order to obtain the best permselectivity, the highest interferences rejection and stability. The procedure of immobilization was proven to be fast and simple and resulted in a better long-term stability and in a higher loading of the enzyme than that obtained by the co-electropolymn. Studies on reproducibility, lifetime of the immobilized enzymes, pH, temp., interferences, buffers, response time, storage and operational time of the biosensors have been carried out. The optimized biosensors allowed rapid quant. assay of cholinesterase inhibitor paraoxon, with a detection limit of 0.1 ppb and a response time of less than ten seconds. (literal)

Editore

• Wiley-VCH - Weinheim (Editore)

Prodotto di

• Materiali e Sistemi nanostrutturati per la bio-sensoristica (PM.P05.004.009) (Modulo)
• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)

Autore CNR

• ANTONELLA CURULLI (Persona)

Insieme di parole chiave

• Parole chiave di "Bienzyme amperometric probes for choline and choline esters assembled with nonconducting electrosynthesized polymers." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)
• Materiali e Sistemi nanostrutturati per la bio-sensoristica (PM.P05.004.009) (Modulo)

Autore CNR di

• ANTONELLA CURULLI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electroanalysis (Rivista)

Editore di

• Wiley-VCH - Weinheim (Editore)

Insieme di parole chiave di

• Parole chiave di "Bienzyme amperometric probes for choline and choline esters assembled with nonconducting electrosynthesized polymers." (Insieme di parole chiave)