Effects of metallic silver particle on resonance energy transfer between fluorophores bound to DNA. (Articolo in rivista)

Type
Label
  • Effects of metallic silver particle on resonance energy transfer between fluorophores bound to DNA. (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Lakowicz J.R., Kusba J., Shen Y., Malicka J., D'Auria S., Grycznyski Z., Grycznyski I. (2003)
    Effects of metallic silver particle on resonance energy transfer between fluorophores bound to DNA.
    in Journal of fluorescence
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lakowicz J.R., Kusba J., Shen Y., Malicka J., D'Auria S., Grycznyski Z., Grycznyski I. (literal)
Pagina inizio
  • 69 (literal)
Pagina fine
  • 77 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 13 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • University of Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology,distances Baltimore, Maryland Technical University of Gdan´sk, Faculty of Applied Physics and Mathematics, (literal)
Titolo
  • Effects of metallic silver particle on resonance energy transfer between fluorophores bound to DNA. (literal)
Abstract
  • We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4’,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Forster distance from 35 Å to an apparent value of 166 Å . These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance. (literal)
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