Articolo in rivista, 2008,

Carbon nanotube-based biosensors.

Ramoni R, Staiano M, Bellucci S, Grycznyski I, Grycznyski Z, Crescenzo R, Iozzino L, Bharill S, Conti V, Grolli S, D’Auria S.

An easy and rapid detection of hazardous compounds is crucial for making on-the-spot irreversible decisions at airport security gates, luggage storage rooms, and other crowded public places, such as stadia, concert halls, etc. In the present study we carried out a preliminary investigation into the possibility of utilizing as advanced nano-biosensors a mutant form of the bovine odorant-binding protein (bOBP) immobilized onto carbon nanotubes. In particular, after immobilization of the protein on the carbon nanotubes we developed a competitive resonance energy transfer (RET) assay between the protein tryptophan residues located at the positions 17 and 133 (W17 and W133) and the 1-amino-anthracene (AMA), a molecule that fits in the binding site of bOBP. The bOBP–AMA complex emitted light in the visible region upon excitation of the Trp donors. However, the addition of an odorant molecule to the bOBP–AMA complex displaced AMA from the binding site making the carbon nanotubes colorless. The results presented in this work are very promising for the realization of a color on/color off b-OBP-based biosensor for the initial indication of hazardous compounds in the environment.

Keywords

CNR authors

Crescenzo Roberta, Iozzino Luisa, D Auria Sabato, Staiano Maria

CNR institutes

IBP – Istituto di biochimica delle proteine