PRIN 2017 - 20174PLLYN_006 - CHIRIACO' MARIA SERENA Prostate cancer: disentangling the relationships with tumor microenvironment to better model and target tumor progression (PRODIGIO) (DFM.AD001.260)

Thematic area

Physical sciences and technologies of matter

Project area

Structure responsible for the research project

Institute of Nanotechnology (NANOTEC)

Project manager

MARIASERENA CHIRIACO
Phone number: 0832319307
Email: mariaserena.chiriaco@nanotec.cnr.it

Abstract

The project aims at establishing an innovative platform to study intrinsic mechanisms and extrinsic signals that define the cross-talk between prostate cancer cells and infiltrating fibroblasts during PCa progression. The platform includes stromal cells culturing from radical prostatectomies (normal and cancer associated fibroblasts), development of 3D organoids from primary PCa cells and 2D non-transformed cell lines, both genetically engineered to recapitulate PCa tumorigenesis. These models will be used to study oncogenic mechanisms due to cell-intrinsic (genetic and epigenetic) and extrinsic (tumor microenvironment) processes, and their interaction during PCa onset and progression.
Validation of key molecular determinants will uncover potential therapeutic targets, and allow the design of lab-on-chip kits as diagnostic tools

Goals

Task 7.1. Real time monitoring of proliferation, motility, invasion.
Task 7.2. On-chip detection of selected proteins from onco-secretome.
Task 7.3. Realization of a prototype for clinical applications.

Start date of activity

29/08/2019

Keywords

Prostate cancer, on chip analysis, secretoma

Last update: 02/01/2025