INDEX - H2020 Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes (DCM.AD007.017)

Area tematica

Scienze chimiche e tecnologie dei materiali

Area progettuale

Struttura responsabile del progetto di ricerca

Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC)

Responsabile di progetto

MARCELLA CHIARI
Telefono: 0228500035
E-mail: marcella.chiari@icrm.cnr.it

Abstract

The aim of Project INDEX is to isolate and characterize nanoparticles available in bodily fluids through development and integration of novel technological breakthroughs. The technology will enable the analysis of clinically valuable nanoparticles called exosomes towards new generation diagnostics. Exosomes are known to mediate communication between cells and their effective utilization holds a great promise of revolutionizing the standard of clinical care. However, their detection and molecular profiling is technically challenging. The proposed technology will isolate exosomes that are as small as 30nm in diameter from human plasma with high purity, and provide in-depth, multi-parameter characterization of the particles through digital counting, size determination, and biological phenotyping.

Obiettivi

Towards this goal: (1) Novel microfluidics will be developed and used for efficient magnetic enrichment; (2) Isolated particles will be detected and analyzed with a novel biological nanoparticle (BNP) sensor (3) Immune-capture and release chemistries as well as phenotyping assays will be developed; (4) Critically, complete on-chip integration of isolation, detection and analysis will be accomplished; (5) Utility of in-depth exosome characterization will be demonstrated with clinical samples for lung cancer.

Data inizio attività

01/10/2017

Parole chiave

Digital detection, exosomes, interferometry

Ultimo aggiornamento: 16/04/2025